MODEST

Mage Oligo Design Tool

Organism Information

Operation Information

Gene Information

Create Oligos

MODEST allows the rapid and effective design of optimal MAGE oligos based on simple input, to achieve any mutation possible with MAGE. Simply input the desired modifications to receive optimized MAGE oligos that will create the modifications along with MASC primer sequences for validation. A manuscript for the software is being prepared.

Oligos created with MODEST is optimal for use with MAGE and recombineering methods where the methyl-directed mismatch repair (MMR) system is unfunctional. Please see online documentation for further details.

To get help, browse the online documentation. To report errors or ideas, please use the icon in the top right of the screen.

Step 1: Choose an organism

You can upload your own GenBank file or choose from GenBank:

Please provide origin of replication (oriC) and termination region:

See the documentation on creating your own genome configuration.
If you have a genome config from a previous run, you can upload that instead of oriC/ter:

Step 2: Input desired DNA modifications

Perform point mutations, insertions, deletions, translational knockouts and expression level modifications. See how in the instructions.

GeneOperation

Load test data set (warning: overrides current data)

Optional step 3: Advanced configuration

Expand/Contract options

(Uncheck this box to force MODEST to center the mutation in all oligos)

Upload or paste a barcoding library. Only needed if barcodes are specified in DNA modifications file. See documentation on barcoding libraries.

Contact info: mskl@cbs.dtu.dk | Version: 0.1.4-2 / 0.2.3