The barcoding file consists of a list of primers followed by a list of barcode ID’s combining a forward and reverse primer. They must follow the headers >PRIMERS and >LIBRARY respectively. The ID’s are used in the gene adjustments file to add the desired barcodes. ID’s should only contain one forward and one reverse primer.
>PRIMERS F1 Sequence F2 Sequence R1 Sequence R2 Sequence .. .. >LIBRARY ID1 F1 R1 ID2 F2 R2 ID3 F1 R1 .. .. ..