Barcoding¶
Barcoding file¶
The barcoding file consists of a list of primers followed by a list of barcode ID’s combining a forward and reverse primer. They must follow the headers >PRIMERS and >LIBRARY respectively. The ID’s are used in the gene adjustments file to add the desired barcodes. ID’s should only contain one forward and one reverse primer.
>PRIMERS
F1 Sequence
F2 Sequence
R1 Sequence
R2 Sequence
.. ..
>LIBRARY
ID1 F1 R1
ID2 F2 R2
ID3 F1 R1
.. .. ..