Barcoding file¶

The barcoding file consists of a list of primers followed by a list of barcode ID’s combining a forward and reverse primer. They must follow the headers >PRIMERS and >LIBRARY respectively. The ID’s are used in the gene adjustments file to add the desired barcodes. ID’s should only contain one forward and one reverse primer.

>PRIMERS
F1  Sequence
F2  Sequence
R1  Sequence
R2  Sequence
..  ..

>LIBRARY
ID1 F1  R1
ID2 F2  R2
ID3 F1  R1
..  ..   ..